MicroLungDetect - Entwicklung und Validierung eines “Lungen-Mikrochips” zur präklinischen Detektion von Medikamenten in der Atemluft

The aim of the interdisciplinary project is to establish a lung microchip for its use in preclinical respiratory gas analysis. The work is a cooperation of the Kluger-Lab and Kemkemer-Lab.

Usually, the dosage of medication is based on body weight or effect. The accompanying measurement of blood concentrations is time-consuming, invasive and expensive. Moreover, the results are only available with after a considerable time delay. The measurement of drug concentrations in the exhaled air represents a promising approach in the individualized medicine and allows to eliminate the previously mentioned disadvantages.

Therefore, an in vitro lung model is urgently needed to investigate the systematic drug transition from "blood" through the tissue into "exhaled air". To build up a physiological and in vivo near alveolar interface, this needs to be simulated using a chip with a membrane and an endothelial-epithelial-co-culture. The focus will be on the formation of a dense and confluent monolayer, as this is a basic prerequisite for the future predictive power of the model. In addition, the model should be long-term stable and persist for at least two weeks in vitro.

Project duration: 03/2018 until 02/2021

Funding: Baden-Württemberg Stiftung

Contact: Petra Kluger, Simon Heine