Epigenetics - Aufbau von 3D gedruckten biomimetischen Konstrukten mit Stammzellen aus dem Fettgewebe zur Identifizierung und Charakterisierung von Typ 2 Diabetes Risiko-Loki auf dem nicht-kodierenden Genom

Genome-wide association studies have identified hundreds of genetic loci associated with Type 2 Diabetes (T2D). However, translating these findings into therapeutics has remained a great challenge. One bottleneck is that the genetic etiology of T2D primarily stems from the non-coding part of the genome, reflected in changes in the epigenome. Non-coding variants impact T2D risk by altering proximal and distal gene regulatory elements, making it difficult to dissect their mechanism of action. The dissection of genetic risk loci is further confounded by the lack of appropriate cell models mimicking microenvironment and three dimensionality of tissues. Such optimized 3D models are more clinically relevant to study gene regulation as culture conditions directly affect the epigenetic state.

The aim of this project is the mechanistic decoding of non-coding risk loci of T2D using additive 3D adipose tissue models.

The work is a cooperation of the Kluger-Lab with Prof. Dr. Melina Claussnitzer from the University of Hohenheim.

Project duration: 01/2019 until 12/2021

Funding: Baden-Württemberg Stiftung

Contact: Petra Kluger, Franziska Atzinger