Fat2Skin - Ein humanes 3-schichtiges Hautmodell als Alternative zum Tierversuch: Verbesserung bisheriger Modelle und Erweiterung des Testspektrums um Feinstaub-assoziierte Substanzen

The human skin represents the major contact area to the environment and a barrier towards harmful external influences. In the chemical, cosmetic and pharmaceutic industry the classification of the toxicity of a substance is mostly made by skin reactions. For lack of adequate alternatives still a host of animal experiments are performed. Due to interspecies variances the transferability of the results to the human system is only possible to a limited extend.

Existing cell-based 2-layered (epidermis and dermis) human in vitro skin models represent a good alternative. However also the subcutaneous adipose tissue (subcutis) plays an essential role in storage and transformation of different substances and influences as an endocrine organ the skin reaction. To enhance the reliability and comparability of such tests with human skin a 3-layered human skin model including the subcutis, is urgently needed. The aim to the project is to establish a 3-layered in vitro human skin model and validate it as testing system. Further the established testing system should be used to investigate the influence of fine dust associated substances on the human skin. 

Project duration: 11/2017 until 10/2019

Funding: Ministerium für Ländlichen Raum und Verbraucherschutz

Contact: Petra KlugerAnn-Cathrin Volz, Freia Schmidt